老司机午夜精品99久久免费,国产色无码专区在线观看,动漫人物桶动漫人物免费观看网站,99精产国品一二三产区

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人正常細(xì)胞>HUVECHUV-EC細(xì)胞, 人臍靜脈內(nèi)皮細(xì)胞

HUV-EC細(xì)胞, 人臍靜脈內(nèi)皮細(xì)胞

簡要描述:HUV-EC-C細(xì)胞, 人臍靜脈內(nèi)皮細(xì)胞
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件

  • 產(chǎn)品型號:HUVEC
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2024-11-16
  • 訪  問  量:2215

產(chǎn)品分類

Product Category

詳細(xì)介紹

HUV-EC-C細(xì)胞, 人臍靜脈內(nèi)皮細(xì)胞

ATCC® Number:CRL-1730™    Price:$280.00
DeBiosSesignations:HUV-EC-C

rum:afety Level:1

Shipped:frozen

Medium &See Propagation

Growth Properties:adherent

Organism:Homo sapiens (human)

Morphology:endothelial

Source:Organ: umbilical vein
Tissue: vascular endothelium
Disease: normal
Cell Type: endothelial


Cellular Products:factor VIII [23284]

Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
HUV-EC-C細(xì)胞, 人臍靜脈內(nèi)皮細(xì)胞

Applications:transfection host (technology from amaxa)

Tumorigenic:No

DNA Profile (STR):Amelogenin: X

CSF1PO: 11,12

D13S317: 9,11

D16S539: 11,12

D5S818: 11,12

D7S820: 8,12

THO1: 6,9.3

TPOX: 8,11

vWA: 16



Cytogenetic Analysis:This is a hypodiploid human cell line. The modal chromosome number was 45 occurring in 72% of cells counted. The rate of polyploid cells was 15.8%. All cells had monosomic N13 and the subclone with additional monosomic N15 predominates. Other coexisting subclones include those with 46,XX,-11,-13,i(11p),i(11q) and 46,XX,+11,-13 karyotypes. Both X chromosomes appear normal.

Comments:Endothelial Cell Growth Supplement (ECGS) and unidentified factors from bovine pituitary, hypothalamus or whole brain extracts are mitogenic for this line. [23284]

The cells have a life expectancy of 50 to 60 population doublings.



Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated of F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: 0.1 mg/ml heparin; 0.03-0.05 mg/ml endothelial cell growth supplement (ECGS); adjust to a final concentration of 10% fetal bovine serum.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C


Subculturing:Protocol:
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37°C.


Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:3 is recommended
Medium Renewal: Two to three times per week


Preservation:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase


Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004

recommended serum:ATCC 30-2020



References:23284: Hoshi H, McKeehan WL. Brain- and liver cell-derived factors are required for growth of human endothelial cells in serum-free culture. Proc. Natl. Acad. Sci. USA 81: 6413-6417, 1984. PubMed: 6333682
29192: Zahedi K. Characterization of the binding of serum amyloid P to laminin. J. Biol. Chem. 272: 2143-2148, 1997. PubMed: 8999915
33021: Soker S, et al. Characterization of novel vascular endothelial growth factor (VEGF) receptors on tumor cells that bind VEGF165 via its exon 7-endoded domain. J. Biol. Chem. 271: 5761-5767, 1996. PubMed:8621443























產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗證碼:

    請輸入計算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
人妻借种太粗进不去| 日韩精品视频一区二区三区| 天天躁日日躁狠狠躁av | 免费a级毛片无码无遮挡| 感受大海的时刻| 日韩a∨精品日韩在线观看| 另类尿喷潮videofree| 精品一区二区三区四区在线观看| 噜噜噜精品欧美成人av| 欧美精品乱码99久久蜜桃| 久亚洲一线产区二线产区三线产区 | 亚洲AV永久无码精品无码麻豆| 亚洲 欧美 日韩 国产 高清| 可播放的中国男gary| JIZZ中国老师高潮喷水| 亚洲精品无码永久在线观看男男| 久久久噜噜噜久久中文字幕色伊伊 | 解开警花的裙子猛烈进入| 一本大道久久东京热无码AV| 普通话丰满的少妇xxxxx野外| 18禁美女黄网站色大片免费看| 丰满大肥婆肥奶大屁股| 永久免费精品精品永久-夜色| 人妻互换亂倫激情| 美女脱了内裤打开腿让男人戳 | 边摸边吃奶又黄激烈高h| 女朋友水太多进去就软了| 国产精品成人69xxx| 一区二区国产精品精华液| 年轻的馊子6中字HD| 国产精品免费精品自在线观看 | 被粗汉h玩松了| 亚洲精品无码永久在线观看男男| 4438全国大成网人网站| 熟女chachacha性少妇| 免费又黄又爽又色的视频| 国产一区日韩二区欧美三区| 19岁女RAPPER潮水欢迎你| 一本一本久久a久久精品综合| 无码AV高潮喷水无码专区线| 学长边洗澡边把我处破了小说|