老司机午夜精品99久久免费,国产色无码专区在线观看,动漫人物桶动漫人物免费观看网站,99精产国品一二三产区

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人腫瘤細(xì)胞、癌細(xì)胞>CRL-5946NCI-H2452細(xì)胞, 人間皮瘤細(xì)胞

NCI-H2452細(xì)胞, 人間皮瘤細(xì)胞

簡要描述:NCI-H2452細(xì)胞, 人間皮瘤細(xì)胞
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞;細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號(hào):CRL-5946
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-16
  • 訪  問  量:1808

產(chǎn)品分類

Product Category

詳細(xì)介紹

NCI-H2452細(xì)胞, 人間皮瘤細(xì)胞

ATCC® Number:CRL-5946™ Price:
Designations:NCI-H2452 [H2452]Depositors:AF Gazdar, JD MinnaBiosafety Level:1Shipped:frozenMedium & Serum:See PropagationGrowth Properties:adherentOrganism:Homo sapiensMorphology:epithelial                         NCI-H2452細(xì)胞, 人間皮瘤細(xì)胞 Source:Disease: mesotheliomaPermits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.Restrictions:The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. A. Gazdar and Dr. J. Minna and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. ephone (214) 699-8056, (214) 688-7233.Isolation:Isolation date: November, 1990DNA Profile (STR):Amelogenin: X,Y CSF1PO: 11,12 D13S317: 12 D16S539: 11,13 D5S818: 11,12 D7S820: 9,11 THO1: 6,9.3 TPOX: 8,11 vWA: 17,18Gender:maleComments:The line was established in November 1990. The tissue donor was a non-smoker.Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.Temperature: 37.0°CSubculturing:Protocol:Remove and discard culture medium.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.Add appropriate aliquots of the cell suspension to new culture vessels.Incubate cultures at 37°C. Subc*tion Ratio: A subc*tion ratio of 1:4 to 1:6 is recommended Medium Renewal: Every 2 to 3 daysPreservation:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phaseRelated Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001recommended serum:ATCC 30-2020References:23570: . NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996..55657: Sekido Y, et al. Neurofibromatosis type 2 (NF2) gene is somatically mutated in mesothelioma but not in lung cancer. Cancer Res. 55: 1227-1231, 1995. PubMed: 788231355700: Penno MB, et al. High CD44 Expression on Human Mesotheliomas Mediates Association with Hyaluronan. Cancer J. Sci. Am. 1: 196-203, 1995.


















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
1吨柴油等于多少升| 亚洲成AV人片一区二区| 无码丰满少妇2在线观看| 天堂中文最新版在线官网在线 | 校花被校长啪到腿软| 国产成人精品综合久久久久| 国产成人久久婷婷精品流白浆| 无码欧精品亚洲日韩一区| 亚洲AV 无码片一区二区三区| 青丝影院视频在线播放| 少妇洁白无删减版178TXT| 年轻护士的滋味中文字幕| 久久久久国产精品嫩草影院欧洲| 亚洲娇小与黑人巨大交| 亚洲男同帅gay片在线观软件| 真实刺激交换娇妻13篇| 黑人玩弄人妻中文在线| 中文字幕无码亚洲字幕成a人 | 日韩人妻无码一区二区三区综合部| 国产老熟女老女人老人| 国产精品毛片一区二区| 极品尤物被啪到呻吟喷水| 国产人成无码视频在线观看| 人人澡人人澡人人看添av| 少妇把腿扒开让我爽爽视频| 最近最新的日本字幕MV| 亚洲av鲁丝一区二区三区| 人人爽久久久噜人人看| 免费真人视频网站| 特黄做受又硬又粗又大视频18| 亚洲 中文 欧美 日韩 在线| 山沟夜晚炕上的呻吟肉版小说| 国产精品无圣光一区二区| 少妇我被躁爽到高潮a片| 2022最新韩国理伦片在线观看| 精品一区二区三区无码视频| 亚洲av人人澡人人爽人人夜夜| 被医生绑在妇科椅调教| 久久96国产精品久久久| a片做爰片仑理片免费看午夜蝴蝶| 日本激情公妇厨房嗯嗯|