老司机午夜精品99久久免费,国产色无码专区在线观看,动漫人物桶动漫人物免费观看网站,99精产国品一二三产区

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人腫瘤細(xì)胞、癌細(xì)胞>CRL-1933769-P細(xì)胞,人腎癌細(xì)胞系

769-P細(xì)胞,人腎癌細(xì)胞系

簡(jiǎn)要描述:769-P細(xì)胞,人腎癌細(xì)胞系
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號(hào):CRL-1933
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2025-04-21
  • 訪  問(wèn)  量:1868

產(chǎn)品分類

Product Category

詳細(xì)介紹

769-P細(xì)胞,人腎癌細(xì)胞系

ATCC® Number:CRL-1933™    Price:$338.00
Designations:769-P

Depositors:RD Williams

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Homo sapiens (human)

Morphology:epithelial


Source:Organ: kidney
Disease: renal cell adenocarcinoma


Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
769-P細(xì)胞,人腎癌細(xì)胞系

Isolation:Isolation date: 1975

Tumorigenic:Yes

DNA Profile (STR):Amelogenin: X

CSF1PO: 11,12

D13S317: 10,14

D16S539: 9,13

D5S818: 12

D7S820: 10,11

THO1: 6,9.3

TPOX: 8,11

vWA: 18



Cytogenetic Analysis:This human cell line contained large numbers of tetra-, hexa-, and higher-ploid cells (2s populations). The modal (s) cell population which occurred in 32% of cells had the pseudodiploid karyotype, 46,XX,-3,-18,del(7) (q21.12;q22.3), ?t(3q?18q). The rate of 2s cells was 42%. Two marker chromosomes, del(7) (q21.12;q22.3) and ?t(3q?18q), were present in all s metaphases, but ?t(3q?18q) was absent in 2s cells. DMs were found in some s cells whereas they were seen in the majority of 2s cells. HSR chromosomes were not found. In s metaphases, both N3 and N18 had only single copy, and the X chromosome was paired.

Age:63 years

Gender:female

Ethnicity:Caucasian

Comments:This line was derived from a primary clear cell adenocarcinoma.

The cells are globular with indistinct borders, have a high nucleus to cytoplasm ratio and exhibit both microvilli and desmosomes.

They can be grown in soft agar.



Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C


Subculturing:Protocol:              
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37°C.


    1. Subc*tion Ratio: A subc*tion ratio of 1:4 to 1:12 is recommended
      Medium Renewal: Every 2 to 3 days



Preservation:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase


Doubling Time:35 hrs

Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020



References:22648: Williams RD, et al. In vitro c*tion of human renal cell cancer. I. Establishment of cells in culture. In Vitro 12: 623-627, 1976. PubMed:1010528
22653: Williams RD, et al. In vitro c*tion of human renal cell cancer. II. Characterization of cell lines. In Vitro 14: 779-786, 1978. PubMed: 721102


















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
japanese日本护士xxx| 18禁裸男晨勃露j毛免费观看| 熟女视频一区二区在线观看| 天美传媒mv免费观看| 亚洲av无码乱码在线观看性色| 精品人妻AV无码一区二区三区| 亚洲 欧美 清纯 校园 另类| 又色又爽又黄的裸体美女图片| 啦啦啦中文免费观看在线| 免费a级毛片做爰片在线| 亚洲成a人片在线观看| 国产av国片免费| 啊┅┅快┅┅用力啊岳| 高撅红肿h羞耻罚老师受学生攻| 免费无码又色又爽的视频软件| 日本xxxx色视频在线播放| 国产免费亂倫在线观看| 漂亮人妻被中出中文字幕久久| 把她抵在试衣间疯狂律动| 免费无码又色又爽的视频软件 | 野外吮她的花蒂两男一女| 露娜拿的金箍棒玩自己视频| 锕锕锕锕锕锕~再深一点五种软件| 解开警花的裙子猛烈进入| 最近中文字幕2019免费版日本| 被调教跪着打屁股| 国色天香一卡2卡三卡4卡乱码| 我的漂亮女房东完整版在线| 色婷婷亚洲精品综合影院| 9I精品福利一区二区三区蜜桃| 丰满岳乱妇在线观看中字无码| 亚洲精品国偷拍自产在线观看蜜臀 | 日本无码欧美一区精品久久| 最近中文字幕2019视频1| 医生边走边吮男男h| xxxx丰满小少妇女高潮| 全部孕妇毛片丰满孕妇孕交| 亚洲av午夜福利精品一区 | 男人桶女人30分钟| 另类 图片 欧美 小说 校园| 国产精品情侣呻吟对白视频|