老司机午夜精品99久久免费,国产色无码专区在线观看,动漫人物桶动漫人物免费观看网站,99精产国品一二三产区

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人腫瘤細(xì)胞、癌細(xì)胞>CRL-2268 BE(2)-C 人神經(jīng)母細(xì)胞瘤細(xì)胞

CRL-2268 BE(2)-C 人神經(jīng)母細(xì)胞瘤細(xì)胞

簡(jiǎn)要描述:CRL-2268 BE(2)-C 人神經(jīng)母細(xì)胞瘤細(xì)胞 ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|。細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號(hào):
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-14
  • 訪  問  量:2278

產(chǎn)品分類

Product Category

詳細(xì)介紹


CRL-2268 BE(2)-C 人神經(jīng)母細(xì)胞瘤細(xì)胞


ATCC® Number:CRL-2268™Price:$329.00
Designations:BE(2)-C

Depositors:JL Biedler

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Homo sapiens (human)

Morphology:neuroblast
CRL-2268 BE(2)-C 人神經(jīng)母細(xì)胞瘤細(xì)胞


Source:Organ: brain

Disease: neuroblastoma

Derived from metastatic site: bone marrow



Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.



Restrictions:BE(2)-C was deposited at the ATCC by June L. Biedler, Memorial Sloan-Kettering Cancer Center. BE(2)C is distributed for academic research purposes only. Memorial Sloan-Kettering releases the line subject to the following: 1.)BE(2)-C or its products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of BE(2)-C including any use by a for-profit entity must first be negotiated with Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; (212) 717-3439.

Isolation:Isolation date: November, 1972

DNA Profile (STR):Amelogenin: X

CSF1PO: 10

D13S317: 11

D16S539: 9,11

D5S818: 12

D7S820: 9,10

THO1: 6

TPOX: 11

vWA: 18



Age:2 years

Gender:male

Comments:BE(2)-C is a clone of the SK-N-BE(2) neuroblastoma cell line (see ATCC CRL-2271) that was established in November of 1972 from a bone marrow biopsy taken from child with disseminated neuroblastoma after repeated courses of chemotherapy and radiotherapy.

BE(2)-C cells have a reported saturation density of greater than 5 X 10 exp5 cells/sq cm.

The cells grow as clusters of flattened neuroblastic cells with occasional fine cell processes (neurites).

Unlike the parent line, they generally do not detach and float.



Propagation:ATCC complete growth medium: The base medium for this cell line is a 1:1 mixture of ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003, and F12 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C



Subculturing:Protocol:
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:4 to 1:6 is recommended

Medium Renewal: Every 2 to 3 days



Preservation:Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase



Doubling Time:18 hrs

Related Products:recommended serum:ATCC 30-2020

parental cell line:ATCC CRL-2271























產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
美国式禁忌2| 人体张悠雨全身光一丝不| 黑人巨大精品欧美一区二区免费| 永久免费a片在线观看全网站| 亚洲av无码一区二区三区观看 | a级毛片高清免费视频就| 99久久久无码国产精品免费砚床| 亚洲AV无码片在线播放| 国产肉体xxxx裸体137大胆| 老汉的性生生活1一7| 久久精品国产99久久丝袜| 小嫩模无套内谢第一次| 久久久久久精品无码人妻| 野花香影院在线观看视频免费| 成人国内精品久久久久一区| 无码人妻丰满熟妇区96| 久久精品国产只有精品66| 强奷人妻日本中文字幕| 被调教跪着打屁股| 久久婷婷五月综合尤物色国产 | 日本黄a级a片国产免费| 激情爆乳一区二区三区| 年轻漂亮的后玛| 日韩+国产| 中文乱幕日产无线码| 少妇沦陷精油按摩中文字幕| 磁力搜索-bt天堂| 亚洲A∨无码一区二区三区| 精品乱码一区二区三区四区| 成人做爰免费观看| 高中生自慰WWW网站| 国产无遮挡裸体免费视频| 国模欢欢销魂全过程| 性xxxxfreexxxxx| 中文字幕无码乱人伦| 一个在上面吃一个在下吃视频| 成年免费视频黄网站在线观看| 解开人妻的裙子猛烈进入| 女士全透毛三角透明内裤| 国产精品扒开腿做爽爽爽A片软件| 韩国午夜理伦三级在线观看中文版|